IMSI (microinjection of morphologically selected sperm) Sperm selection is carried out at 8,000 magnification by this technique, allowing better sperm nucleus assessment. Adapted to injection, it takes the name intracytoplasmic morphologically selected sperm injection (IMSI). The first results published suggest that IMSI allows higher implantation and pregnancy rates, especially after two ICSI failures.The sperm selection by the morphology is considered one of the best indicators for achieving successful fertilization and assisted reproduction 


Gris Reproducción-CIRH lab is first Embryology Laboratory of Spain that counts on this novel technique

Induction of meiotic and post-meiotic alterations of male germ cells in vitro has been the target of several research efforts since 1960. However, to date, the establishment of an ideal culture system in which spermatogonial stem cells can be maintained and directed to proliferate and undergo meiosis and complete spermiogenesis does not exist. This is attributed to the difficulties concerning the isolation and purification of defined subpopulations of germ cells and the establishment of male germ cell lines. In addition, there is no adequate knowledge regarding the optimal biochemical conditions that promote the survival and differentiation of germ cells in long-term cultures. This review focuses on the methodologies that have been proved sufficient to achieve differentiation of cultured male germ cells. Furthermore, the factors regulating spermatogenesis and the technical prerequisites to achieve differentiation of cultured male germ cells are described. Finally, the role of in vitro cultures of immature diploid germ cells in the therapeutic management of men negative for haploid cells in their testes and the subsequent potential genetic and epigenetic risks are discussed.

There are several possibilities to preserve future fertility in women exposed to chemotherapy: in vitro fertilization (IVF) and embryo cryopreservation, ovarian tissue cryopreservation, unfertilized ova cryopreservation, and the administration of a  gonadotropin-releasing hormone (GnRH) agonist.